|
|
|
|
|
Journal |
Textile Coloration and Finishing |
Year / Vol., Issue, Month |
2018 / V.30,no.4,Dec |
Title |
Immobilization of Lysozyme from Hen Egg by Crosslinking Method onto Chitosan Non-woven |
Authors |
So Hee Lee* |
Institution/Affiliations |
Gyeongsang National University |
Pages/Total page |
pp.264 ~ 274 / 11 |
Language |
Korean |
Abstract |
Abstract Immobilization of lysozyme on chitosan non-woven using glutaraldehyde(GA)
was investigated. For this, 100 % chitosan non-woven was prepared as novel support for
the enzyme immobilization. In addition, free lysozyme activity was examined depending
on various pH and temperature by measuring time. Moreover, the optimum immobilization conditions depending on various pH, temperature, immobilization time and lysozyme
concentration was evaluated. In addition, thermal stability and storage stability of immobilized lysozyme were measured. The characteristics of immobilized lysozyme was examined
by FT-IR, surface morphology, and MTT assay. The results are follows: the optimal
immobilization of lysozyme were pH 7.0, 25 ¡É, lysozyme concentration 1.5 mg/ml, immobilization time 240 min. The immobilized lysozyme showed higher thermal stability
than the free trypsin. The immobilized lysozyme activity was retained 80 % of its initial
activity at 4 ¡É over 30 days of storage. The lysozyme was immobilized effectively on
chitosan non-woven by observation of surface morphology. |
Keywords |
Keywords immobilization, chitosan, non-woven, lysozyme, glutaraldehyde |
Download |
|
Reference |
http://www.koreascience.or.kr/article/ArticleFullRecord.jsp?cn=OSGGBT_2018_v30n4_264&ordernum= |
|
|
|
|
|
|
|
|
|