Journal Textile Coloration and Finishing Year / Vol., Issue, Month 2018 / V.30,no.4,Dec Title Immobilization of Lysozyme from Hen Egg by Crosslinking Method onto Chitosan Non-woven Authors So Hee Lee* Institution/Affiliations Gyeongsang National University Pages/Total page pp.264 ~ 274 / 11 Language Korean Abstract Abstract Immobilization of lysozyme on chitosan non-woven using glutaraldehyde(GA) was investigated. For this, 100 % chitosan non-woven was prepared as novel support for the enzyme immobilization. In addition, free lysozyme activity was examined depending on various pH and temperature by measuring time. Moreover, the optimum immobilization conditions depending on various pH, temperature, immobilization time and lysozyme concentration was evaluated. In addition, thermal stability and storage stability of immobilized lysozyme were measured. The characteristics of immobilized lysozyme was examined by FT-IR, surface morphology, and MTT assay. The results are follows: the optimal immobilization of lysozyme were pH 7.0, 25 ℃, lysozyme concentration 1.5 mg/ml, immobilization time 240 min. The immobilized lysozyme showed higher thermal stability than the free trypsin. The immobilized lysozyme activity was retained 80 % of its initial activity at 4 ℃ over 30 days of storage. The lysozyme was immobilized effectively on chitosan non-woven by observation of surface morphology. Keywords Keywords immobilization, chitosan, non-woven, lysozyme, glutaraldehyde Download   Reference http://www.koreascience.or.kr/article/ArticleFullRecord.jsp?cn=OSGGBT_2018_v30n4_264&ordernum=