|
|
|
|
|
 |
 년도별 목차보기 |
| * 원문 PDF 파일은 로그인 하시기 바랍니다. |
 |
|
| 발행년도/권호 |
2018 년 / 권호: V.30,no.4,Dec |
| 논문제목1(원어) |
키토산 섬유를 담체로 이용한 라이소자임 효소의 고정화 |
| 논문제목2(타언어) |
Immobilization of Lysozyme from Hen Egg by Crosslinking Method onto Chitosan Non-woven |
| 저자(원어) |
이소희* |
| 저자(타언어) |
So Hee Lee* |
| 소속(원어) |
경상대학교 의류학과/기초과학연구소 |
| 소속(타언어) |
Gyeongsang National University |
| 시작/끝
페이지 |
264 ~ 274 총페이지 : 11 |
| 언어(원어) |
Korean |
| 초록 |
Abstract Immobilization of lysozyme on chitosan non-woven using glutaraldehyde(GA)
was investigated. For this, 100 % chitosan non-woven was prepared as novel support for
the enzyme immobilization. In addition, free lysozyme activity was examined depending
on various pH and temperature by measuring time. Moreover, the optimum immobilization conditions depending on various pH, temperature, immobilization time and lysozyme
concentration was evaluated. In addition, thermal stability and storage stability of immobilized lysozyme were measured. The characteristics of immobilized lysozyme was examined
by FT-IR, surface morphology, and MTT assay. The results are follows: the optimal
immobilization of lysozyme were pH 7.0, 25 ℃, lysozyme concentration 1.5 mg/ml, immobilization time 240 min. The immobilized lysozyme showed higher thermal stability
than the free trypsin. The immobilized lysozyme activity was retained 80 % of its initial
activity at 4 ℃ over 30 days of storage. The lysozyme was immobilized effectively on
chitosan non-woven by observation of surface morphology. |
| 키워드 |
Keywords immobilization, chitosan, non-woven, lysozyme, glutaraldehyde |
| 학회지 |
|
| Reference |
http://www.koreascience.or.kr/article/ArticleFullRecord.jsp?cn=OSGGBT_2018_v30n4_264&ordernum= |
|
 |
 |
 |
|
|
|
|
간행물정보
한국염색가공학회지
