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| 발행년도/권호 |
2018 년 / 권호: V.30,no.2,Jun |
| 논문제목1(원어) |
미생물 합성에 의한 poly(3-hydroxybutyrate)의 분자량과
말단관능기 제어 |
| 논문제목2(타언어) |
Control of Molecular Weight and Terminal Groups of Poly
(3-hydroxybutyrate) in Bio-synthesis |
| 저자(원어) |
이찬우 |
| 저자(타언어) |
Chan Woo Lee |
| 소속(원어) |
호서대학교 바이오산업학부 |
| 소속(타언어) |
Hoseo University |
| 시작/끝
페이지 |
130 ~ 140 총페이지 : 11 |
| 언어(원어) |
Korean |
| 초록 |
Abstract In the bio-synthesis of poly(3-hydroxybutyrate)(PHB), which is a kind of
poly(3-hydroxyalkanoate)(PHA), aimed to control the low molecular weight of PHB and
obtain a telechelic PHB. As a result of incubation of R. eutropha at 30℃ with ethylene
glycol added as a chain transfer agent, PHB content on the dry cell weight increased up
to 24h, however, it decreased after that, and the molecular weight of PHB increased from
9h to 12h, and then, decreased up to 72h. The decrease of the content and the molecular
weight of PHB indicates that PHB was decomposed as an energy source in bacterial cells
and was incorporated into metabolic pathways. 1H-NMR of the obtained PHB after incubation
for 72h was measured to determine the terminal groups of the PHB during incubation.
As the results of １H-NMR measurement, the peaks derived from ethylene glycol
in both terminals of PHB were observed. Which indicate that the terminal reaction was
caused by the addition of ethylene glycol, and that telechelic PHB having hydroxyl group
at the both terminals where molecular weight was controlled was successfully synthesized. |
| 키워드 |
Keywords bio-synthesis, low molecular weight, terminal groups, telechelic, poly(3-hydroxybutyrate),
energy source, chain transfer agent |
| 학회지 |
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| Reference |
http://www.koreascience.or.kr/article/ArticleFullRecord.jsp?cn=OSGGBT_2018_v30n2_130&ordernum= |
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