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| 발행년도/권호 |
2007 년 / 권호: V.19,no.4,Aug |
| 논문제목1(원어) |
1,3-bisdicyanovinylindane을 이용한 단백질 패터닝의 광학적 감지 |
| 논문제목2(타언어) |
Optical detection of protein patterns using 1,3-bisdicyanovinylindane |
| 저자(원어) |
박영민․이지혜1․이창수1․손영아 |
| 저자(타언어) |
Young-Min Park, Ji-Hye Lee1, Chang-Soo Lee1 and Young-A Son |
| 소속(원어) |
충남대학교 |
| 소속(타언어) |
Chungnam National University |
| 시작/끝
페이지 |
32 ~ 37 총페이지 : 6 |
| 언어(원어) |
Korean |
| 초록 |
In this study, we have obtained the protein patterns using the membrane patterning of
soft-lithography technique. The rapid detection of protein including bovine serum albumin (BSA) was resulted
from the interaction with 1,3-bisdicyanovinylindane. For the proof of the interaction between BSA and dye, the
UV-vis absorption spectra of BSA and dye were observed at 278 nm and 580 nm, respectively. As expected, the
absorption spectrum of the interaction between BSA and dye was observed at 584nm. The absorption spectrum
of the interaction was red-shifted. In addition, the optical images of the selectively reacted protein patterns
showed the distinctive change of patterned color at different pH conditions. Because the dye has negative
charges, the charge of BSA at different pH conditions could influence the interaction behavior between dye and
BSA. Therefore, in the case of pH 7, the selectively patterned protein substrates obtained deep blue color pattern
caused by electrostatic interaction between negative charges of the dye and positive charges of the BSA.
However, in the case of pH 10, selectively patterned protein substrates obtained light blue color pattern because
the electrostatic interaction was relatively lower than pH 7 due to the change of overall charge distribution of
BSA. |
| 키워드 |
1,3-bisdicyanovinylindane, Soft-lithography, Bovine serum albumin(BSA), Patterning |
| 학회지 |
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| Reference |
http://www.koreascience.or.kr/article/ArticleFullRecord.jsp?cn=OSGGBT_2007_v19n4_32 |
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